The molecular mass ( m) is the mass of a given molecule. The unit dalton (Da) is often used. Different molecules of the same compound may have different molecular masses because they contain different of an element. The derived quantity relative molecular mass is the unitless ratio of the mass of a molecule to the atomic mass constant (which is equal to one dalton).IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"
The molecular mass and relative molecular mass are distinct from but related to the molar mass. The molar mass is defined as the mass of a given substance divided by the amount of the substance, and is expressed in grams per mol (g/mol). That makes the molar mass an average of many particles or molecules (potentially containing different Isotope), and the molecular mass the mass of one specific particle or molecule. The molar mass is usually the more appropriate quantity when dealing with macroscopic (weigh-able) quantities of a substance.
The definition of molecular weight is most authoritatively synonymous with relative molecular mass; however, in common practice, use of this terminology is highly variable. When the molecular weight is given with the unit Da, it is frequently as a weighted average similar to the molar mass but with different units. In molecular biology, the mass of macromolecules is referred to as their molecular weight and is expressed in kDa, although the numerical value is often approximate and representative of an average.
The terms "molecular mass", "molecular weight", and "molar mass" may be used interchangeably in less formal contexts where unit- and quantity-correctness is not needed. The molecular mass is more commonly used when referring to the mass of a single or specific well-defined molecule and less commonly than molecular weight when referring to a weighted average of a sample. Prior to the 2019 redefinition of SI base units quantities expressed in daltons (Da) were by definition numerically equivalent to molar mass expressed in the units g/mol and were thus strictly numerically interchangeable. After the 20 May 2019 redefinition of units, this relationship is only nearly equivalent, although the difference is negligible for all practical purposes.
The molecular mass of small to medium size molecules, measured by mass spectrometry, can be used to determine the composition of elements in the molecule. The molecular masses of macromolecules, such as proteins, can also be determined by mass spectrometry; however, methods based on viscosity and light-scattering are also used to determine molecular mass when crystallographic or mass spectrometric data are not available.
Atomic and molecular masses are usually reported in daltons, which is defined in terms of the mass of the isotope 12C (carbon-12). Relative atomic and molecular masses as defined are dimensionless. However, the name unified atomic mass unit (u) is still used in common practice. For example, the relative molecular mass and molecular mass of methane, whose molecular formula is CH4, are calculated respectively as follows:
The uncertainty in molecular mass reflects variance (error) in measurement not the natural variance in isotopic abundances across the globe. In high-resolution mass spectrometry the mass isotopomers 12C1H4 and 13C1H4 are observed as distinct molecules, with molecular masses of approximately 16.031 Da and 17.035 Da, respectively. The intensity of the mass-spectrometry peaks is proportional to the isotopic abundances in the molecular species. 12C 2H 1H3 can also be observed with molecular mass of 17 Da.
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Calculation
12.00 4.0313 16.0313
Determination
Mass spectrometry
Mass photometry
detecting protein oligomerisation state, characterisation of complex macromolecular assemblies (ribosomes, GroEL, AAV) and protein interactions such as protein-protein interactions.Soltermman et al. Quantifying protein-protein interactions by molecular counting using mass photometry. Angew. Chem Int Ed, 2020, 59(27), 10774-10779 Mass photometry can measure molecular mass to an accurate degree over a wide range of molecular masses (40kDa – 5MDa).
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