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Rhodamine
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Rhodamine is a family of related dyes, a subset of the triarylmethane dyes. They are derivatives of . Important members of the rhodamine family are rhodamine 6G, , (Sulforhodamin 101), rhodamine 123, and . They are mainly used to dye paper and inks, but they lack the for fabric dyeing.


Use
Aside from their major applications, they are often used as a , e.g. to determine the rate and direction of flow and transport of water. Rhodamine dyes and can thus be detected using . Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, , fluorescence correlation spectroscopy and . Rhodamine 123 is used in biochemistry to inhibit function. Rhodamine 123 appears to bind to the mitochondrial membranes and inhibit transport processes, especially the electron transport chain, thus slowing down cellular respiration. It is a substrate of (Pgp), which is usually overexpressed in cancer cells. Recent reports indicate that rhodamine 123 may also be a substrate of multidrug resistance-associated protein (MRP), or more specifically, MRP1.

In addition to their major applications, rhodamines are used in as gain media.F. P. Schäfer (Ed.), Dye Lasers, 3rd Ed. (Springer-Verlag, Berlin, 1990).F. J. Duarte and L. W. Hillman (Eds.), Dye Laser Principles (Academic, New York, 1990).


Other derivatives
There are many rhodamine derivatives used for imaging purposes, for example Carboxytetramethylrhodamine (TAMRA), tetramethylrhodamine (TMR) and its isothiocyanate derivative (TRITC) and sulforhodamine 101 (and its sulfonyl chloride form ) and Rhodamine Red. TRITC is the base rhodamine molecule functionalized with an group (−N=C=S), replacing a atom on the bottom ring of the structure. This derivative is reactive towards groups on proteins inside cells. A succinimidyl-ester functional group attached to the rhodamine core, creating NHS-rhodamine, forms another common amine-reactive derivative.

Other derivatives of rhodamine include newer fluorophores such as Alexa 546, Alexa 633, , HiLyte fluor 555 HiLyte 594, Janelia Dyes JF549 and JF669 have been tailored for various chemical and biological applications where higher , increased brightness, different spectral characteristics, or different attachment groups are needed.

Substituents of the core are influencing the properties of the dyes by both electronic and steric effects. Specifically designed also allows xanthenes to bear specific functions activatable upon excitation by , e.g. they could act as photoremovable protecting group for and , (thus being a photoCORM), or added as a secondary functionality of fluorescent dyes, e.g. fluorescent .


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