Ligation may refer to:
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Ligation (molecular biology), the covalent linking of two ends of DNA or RNA molecules
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Chemical ligation, the chemoselective condensation of unprotected peptides
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In medicine, the making of a ligature (tie)
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Tubal ligation, a method of female sterilization
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Rubber band ligation, a treatment for hemorrhoids
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In coordination chemistry, making a bond between a ligand and a Lewis acid
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In orthodontics, a method of attaching the archwires to the brackets
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KAHA Ligation
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Ligation-independent cloning
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Typographic ligature forming
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