Peropsin, a visual pigment-like receptor, is a protein that in humans is encoded by the RRH gene.[ Material was copied and adapted from this source, which is available under a Creative Commons Attribution 4.0 International License.]
Photochemistry
Like most opsins, peropsins have in its seventh transmembrane domain a
lysine corresponding to
amino acid position 296 in
cattle rhodopsin,
which is important for retinal binding and light sensing.
In amphioxus, a cephalochordate, a peropsin binds in the dark-state all-trans-retinal instead of 11-cis-retinal,
Tissue localization
In mice, a peropsin is localized to the apical
microvilli of the retinal pigment epithelium (RPE).
There, it regulates storage or the movement of
vitamin A from the
retina to the RPE.
A peropsin is also expressed in
of the human
skin. In keratinocyte
cell culture, it reacts to
ultraviolet if retinal is supplied.
In
chicken, a peropsin is expressed with an RGR-opsin in the
pineal gland and the retina.
Gene localization and structure
The human peropsin gene lies on
chromosome 4 band 4q25 and has six
like RGR-opsins. However only two of these introns are inserted at the same place, which still indicates that peropsins and RGR-opsins are more closely related to each other than to the ciliary and rhabdomeric opsins.
This shared gene structure is also reflected in opsin phylogenies, where peropsins and RGR-opsins are in the same group: The chromopsins.
Phylogeny
The peropsins are restricted to the
and the cephalochordates.
The
are the
taxon that contains
and with them humans. The peropsins are one of the seven subgroups of the chromopsins. The other groups are the RGR-opsins, the
, the nemopsins, the astropsins, the varropsins, and the gluopsins.
The chromopsins are one of three subgroups of the tetraopsins (also known as RGR/Go or Group 4 opsins). The other groups are the neuropsins and the Go-opsins. The tetraopsins are one of the five major groups of the animal
, also known as type 2 opsins). The other groups are the ciliary opsins (c-opsins, cilopsins), the rhabdomeric opsins (r-opsins, rhabopsins), the xenopsins, and the nessopsins. Four of these subclades occur in
Bilateria (all but the nessopsins).
However, the bilaterian clades constitute a
paraphyly taxon without the opsins from the
.
In the phylogeny above, Each clade contains sequences from opsins and other G protein-coupled receptors. The number of sequences and two pie charts are shown next to the clade. The first pie chart shows the percentage of a certain amino acid at the position in the sequences corresponding to position 296 in cattle rhodopsin. The amino acids are color-coded. The colors are red for lysine (K), purple for glutamic acid (E), orange for arginine (R), dark and mid-gray for other amino acids, and light gray for sequences that have no data at that position. The second pie chart gives the taxon composition for each clade, green stands for , dark green for , mid green for , brown for , pale pink for , dark blue for , light blue for , and purple for . The branches to the clades have pie charts, which give support values for the branches. The values are from right to left SH-aLRT/aBayes/UFBoot. The branches are considered supported when SH-aLRT ≥ 80%, aBayes ≥ 0.95, and UFBoot ≥ 95%. If a support value is above its threshold the pie chart is black otherwise gray.
Clinical significance
Since RGR-opsin may be associated with retinitis pigmentosa,
which is like peropsin also expressed in the retinal pigment epithelium, peropsin was screened for a link with retinitis pigmentosa.
However, no link could be established.
