Lys-N is a metalloendopeptidase found in the mushroom
Grifola frondosa that cleaves proteins on the amino side of
lysine residues.
[Image:N-Lys1.png|thumb|right|Crystal]]
Mass spectrometry
Lys-N is becoming a popular protease used for protein digestion in
proteomics experiments. The combination Lys-N
proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra composed mostly of amino terminal peptide fragment ions.
This fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing.
See also
-
Endoproteinase Lys-C
-
Mass spectrometry
-
Tandem mass spectrometry
-
Bottom-up proteomics
External links
