GE: electrophoresis chamber,
peristaltic pump, fraction collector, buffer recirculation pump and UV detector (in the
refrigerator),
power supply and recorder (on the table).
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Electroelution is a method used to extract a
nucleic acid or a
protein sample from an
electrophoresis gel by applying a negative
Electric current in the plane of the smallest dimension of the gel, drawing the
macromolecule to the surface for extraction and subsequent analysis.
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]
For example,
electroblotting is based upon the same principle.
DNA extraction
Using this method,
DNA fragments can be recovered from a particular region of
agarose or
polyacrylamide gels. The gel piece containing the fragment is excised (cut out from the whole gel) and placed in a dialysis bag with
Buffer solution. Electrophoresis causes the DNA to migrate out of the gel into the dialysis bag buffer. The DNA fragments are recovered from this buffer and purified, using phenol–chloroform extraction followed by ethanol precipitation. This method is simple, rapid and yields high recovery (75%) of DNA fragments from gel pieces.
Protein extraction
QPNC-PAGE yields more than 95% recovery of
. In this process, proteins are continuously
Elution from a gel column and separated in different fractions according to their isoelectric point.
