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14.3.1 Reagents and Supplies Allow approximately 1 h for setup, 4 h for reaction, and 6 h for dialysis or 1 h for gel filtration. No special equipment is needed. Prepare the following reagents and other materials in advance. RITC solution: ...
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Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications. Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.
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