All cells must balance pools of coenzyme A (CoA) and its thioesters.
All cells must balance pools of coenzyme A (CoA) and its thioesters. AMP-forming acyl-CoA synthetases contribute to this process by activating carboxylic acids to their corresponding acyl-CoA thioesters. These enzymes are regulated by a sirtuin-dependent protein acylation/deacylation system (SDPADS). The SDPADS of Salmonella enterica consists of a protein acetyltransferase (Pat) and an NAD -dependent deacetylase, or sirtuin (CobB).