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The use of vaccinia virus E3L amino-terminal mutants in viral oncolysis and characterization in the murine JC cell line
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EAN 2940011776298
REGISTERED: 11/21/17
UPDATED: 10/25/21
The use of vaccinia virus E3L amino-terminal mutants in viral oncolysis and characterization in the murine JC cell line.
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While vaccinia virus (VACV) is commonly known for its role as a vaccine for smallpox, much research has been directed at its potential use as viral oncolytic agent, capable of lysing cancerous cells and destroying tumors. By creating mutations in the VACV E3L gene, an inhibitor of the double stranded protein kinase R, several viruses have been developed that preferentially replicate in a number of human cancer cell lines. These results demonstrate that viruses with a particular deletion in the


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  • EAN bar code 2940011776298 ξ1 registered March 16 2016
  • Product category is The-use-of-vaccinia-virus-E3L-amino-terminal-mutants-in-viral-oncolysis-and-characterization-in-the-murine-JC-cell-line Kelly-Lee-Trainor Electronic Cell

While vaccinia virus (VACV) is commonly known for its role as a vaccine for smallpox, much research has been directed at its potential use as viral oncolytic agent, capable of lysing cancerous cells and destroying tumors. By creating mutations in the VACV E3L gene, an inhibitor of the double stranded protein kinase R, several viruses have been developed that preferentially replicate in a number of human cancer cell lines. These results demonstrate that viruses with a particular deletion in the amino-terminus of E3L (E3Ldel54N) are non-pathogenic at high titers in a severe combined immunodeficient (SCID) mouse model. Additionally, it is shown that in a SCID/beige mouse xenogaft model, E3Ldel54N mutants are capable of causing tumor regression in an infected tumor at a low dose of virus, and when tumors are infected at a high dose the virus is capable spreading to distant, uninfected tumors, causing regression. An important step in developing safe and effective viral therapeutics and vaccines is to understand the mode of viral pathogenesis at the molecular level. Assays using animal models to study these interactions can be costly and complicated; therefore a cell culture model is desired. In this work it is shown for the first time the necessity of the E3L amino terminus in VACV replication in a cell culture model using a murine mammary epithelial adenocarcinoma (JC) cell line. Furthermore, using the JC cell line, it is possible to reveal the mechanisms underlying the inhibition of virus replication and demonstrated that replication of VACV is linked to Z-form nucleic acid binding, which also correlates with viral pathogenesis in the animal. Using microarray analysis, differences were identified in gene expression profiles between VACV infected JC and HeLa cells, as well as showed changes in expression between wild type VACV and E3L mutants within the JC cell line.


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    ^ The use of vaccinia virus E3L amino-terminal mutants in viral oncolysis and characterization in the murine JC cell line. (revised Oct 2021)

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